期刊：BMCPlantBiology
主题：油菜素内酯促*瓜根系铵硝吸收机制
标题：24-EpibrassinolidepromotesNO3-andNH4+ionfluxrateandNRT1geneexpressionincucumberundersuboptimalrootzonetemperature
影响因子：3.
检测指标：NO3-、NH4+流速
检测部位：根毛区
作者：中国农业科学院蔬菜与花卉研究所AliAnwar、李衍素、于贤昌
英文摘要
Suboptimalrootzonetemperature(RZT)causesaremarkablereductioningrowthofhorticulturalcropsduringwintercultivationundergreenhouseproduction.However,limitedinformationisavailableontheeffectsofsuboptimalRZTonnitrogen(N)metabolismincucumberseedlings.Theaimofthisstudyistoinvestigatetheeffectsof24-Epibrassinolide(EBR)onnitrateandammoniumfluxrate,Nmetabolism,andtranscriptlevelsofNRT1familygenesundersuboptimalRZTincucumberseedlings.
SuboptimalRZT(LT)negativelyaffectedoncucumbergrowthandproportionatelydecreasedEBRcontents,bleedingrate,rootactivity,enzymeactivitiesofnitratereductase(NR),nitritereductase(NiR),glutaminesynthetase(GS),andglutamatesynthase(GOGAT),nitrate(NO3)influxrate,ammonium(NH4+)effluxrate,andtranscriptlevelsofnitratetransporter(NRT1)encodinggenes.However,exogenousEBRreducedtheharmfuleffectsofsuboptimalRZTandincreasedendogenousEBRcontents,bleedingrate,rootactivity,enzymeactivitiesofNR,NiR,GS,andGOGAT,NH4+andNO3fluxratesandcontents,andNaccumulation.EBR-treatedseedlingsalsoupregulatedthetranscriptlevelsofnitratetransportersCsNRT1.1,CsNRT1.2A,CsNRT1.2B,CsNRT1.2C,CsNRT1.3,CsNRT1.4A,CsNRT1.5B,CsNRT1.5C,CsNRT1.9,andCsNRT1.10,anddownregulatedCsNRT1.5AandCsNRT1.8.LTtreatmentupregulatedtheexpressionlevelofCsNRT1.5A,whileexogenousBZRapplicationdownregulatedtheexpressionlevelofNRT1genes.TheseresultsindicatethatexogenousapplicationofEBRalleviatedtheharmfuleffectsofsuboptimalRZTthroughchangesinNmetabolism,NH4+andNO3fluxrates,andNRT1geneexpression,leadingtoimprovedcucumberseedlingsgrowth.OurstudyprovidesthefirstevidenceoftheroleofEBRintheresponsetosuboptimalRZTincucumber,andcanbeusedtoimprovevegetableproduction.
中文摘要（谷歌机翻）
次优根区温度（RZT）导致在温室生产期间冬季栽培期间园艺作物的生长显着减少。然而，关于次优RZT对*瓜幼苗中氮（N）代谢的影响的信息有限。本研究的目的是研究24-表油菜素内酯（EBR）对*瓜幼苗次优RZT下NRT1家族基因的**盐和铵通量，N代谢和转录水平的影响。次优RZT（LT）对*瓜生长有负面影响，并且比例降低EBR含量，出血率，根系活力，**还原酶（NR），亚**还原酶（NiR），谷氨酰胺合成酶（GS）和谷氨酸合成酶（GOGAT）的酶活性，**盐（NO3-）流入速率，铵（NH4+）流出速率和**盐转运蛋白（NRT1）编码基因的转录水平。然而，外源EBR降低了次优RZT的有害作用，增加了内源EBR含量，出血率，根系活力，NR，NiR，GS和GOGAT的酶活性，NH4+和NO3-通量率和含量以及N积累。EBR处理的幼苗也上调了**盐转运蛋白CsNRT1.1，CsNRT1.2A，CsNRT1.2B，CsNRT1.2C，CsNRT1.3，CsNRT1.4A，CsNRT1.5B，CsNRT1.5C，CsNRT1.9和CsNRT1的转录水平。.10，并下调CsNRT1.5A和CsNRT1.8。LT处理上调CsNRT1.5A的表达水平，而外源BZR应用下调NRT1基因的表达水平。这些结果表明，外源施用EBR通过改变N代谢，NH4+和NO3-通量率以及NRT1基因表达减轻了次优RZT的有害作用，从而改善了*瓜幼苗的生长。我们的研究首次证明了EBR在*瓜中对次优RZT的反应中的作用，并可用于改善蔬菜生产。
Fig.4EffectsofsuboptimalRZTandEBRonNO3-(A;scatterNO3-fluxrate,B;averageNO3-fluxrate)fluxrateinrootsofcucumberseedlingsundersuboptimalRZTtreatment.Fluxratewasrecordedfor10mininrootssevendaysaftertreatment.Eachpointisthemeanofnineindividualseedlingsandbarsindicatestandarddeviations.Treatmentswiththesamelettersarenotsignificantlydifferentbytheleastsignificantdifference(LSD)testatP=0.05